Real Time PCR Master Mixes
Selleck Chemical LLC 2x SYBR Green qPCR Master Mix
Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity specIFicity and reliability compared with Roche Abi and Takara offered at lowest price on the market
Biotium Forget-Me-Not™ EvaGreen qPCR Master Mix (2-Color Tracking) (2000 reactions)
Forget-Me-Not™ EvaGreen qPCR Master Mix is a hot-start EvaGreen dye-based master mix for use in real time PCR applications and DNA melt curve analysis. Forget-Me-Not™ features a unique combination of a master mix containing a low concentration of blue dye, plus a DNA template buffer containing a higher concentration of blue dye. This 2-color tracking system allows you to see at a glance whether you forgot to add master mix or template to any of your reactions, so you can catch pipetting mistakes and avoid wasting time, reagents, and your precious DNA samples. The master mix features EvaGreen Dye which is suitable for HRM analysis and is safer, more environmentally friendly, and more sensitive than SYBR Green. The kit also features Cheetah™ Hotstart Taq for fast cycling protocols. This product has sufficient reagent for 2000 PCR reactions.
New England Biolabs, Inc. Quick-Load Taq 2X Master Mix – 500 reactions (50 µl vol)
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. Quick-Load Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl, tracking dyes and stabilizers. The presence of two commonly used tracking dyes for DNA gels, Orange G and Xylene Cyanol FF, gives the master mix a green color, allowing direct loading of the PCR product onto agarose gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at approximately 4 kb and Orange G migrates at approximately 50 bp. The amount of tracking dyes included does not mask co-migrating DNA bands. Quick-Load Taq 2X Master Mix is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
Roche Diagnostics FS ESSENTIAL DNA GREEN MASTER
FastStart Essential DNA Green Master Ready-to-use hot start reaction mix for SYBR Green I-based real-time PCR using the LightCycler 96 Instrument and the LightCycler Nano Instrument. 5 x 1 ml for up to 500 reactions of 20 l final volume
ABclonal Technology BrightCycle Universal SYBR Green qPCR Mix with UDG
This product is a universal SYBR Green reagent that uses a specially designed reference dye (ROX), has a more sensitive resolution, and can be applied to all types of fluorescence quantitative PCR instruments on the market (including High ROX, Low ROX, and No ROX required instruments). This reagent introduces a dUTP/UDG anti fouling system. UDG can quickly degrade pollutants containing U at room temperature, and UDG quickly deactivates when pre denatured at 95 ℃, without affecting the efficiency and sensitivity of qPCR. This product uses hot start Taq DNA polymerase for amplification, which greatly improves the specificity of the product while ensuring the amplification effect.
Roche Diagnostics MULTIPLEX RNA VIRUS MASTER
LightCycler Multiplex RNA Virus MasterOne-step RT-qPCR Master Mix for sensitive and reliable multiplex detection of viral RNA targets and internal controls.1000 x 20 L reactions
Sigma Aldrich Fine Chemicals Biosciences fastStart Universal SYBR G
fastStart(TM) Universal SYBR(R) Green Master (Rox) is a ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX. SYBR(R) Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis the SYBR(R) Green I dye which is included in the reaction mix binds to the amplified PCR products. The amplicon can be detected by its fluorescence. Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart(TM) Taq DNA Polymerase make the modified enzyme inactive at room temperature (15 to 25C). Therefore there is no elongation during the period when primers can non-specifically bind. fastStart(TM) Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e. a pre-incubation step at 95C).
New England Biolabs, Inc. AhdI – 5000 units
A restriction endonuclease that recognizes the sequence GACNN_N^NNGTC.
New England Biolabs, Inc. Luna Universal qPCR Master Mix – 500 rxn (5 x 1 ml)
The NEB Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used.
New England Biolabs, Inc. WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) – 100 reactions (25 µl vol)
The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15-40 minutes.
ABclonal Technology ABScript Neo RT Master Mix for qPCR with gDNA Remover
ABScript Neo RT Master Mix for qPCR with gDNA Remover is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H2O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.
Biotium Fast Probe Master Mix with Rox (500 rxn)
Fast Probe Master Mix is formulated for conducting real-time PCR using an oligo-based probe, such as a TaqMan probe, a MGB probe or an AllGlo™ probe. The master mix contains buffer, dNTPs, MgCl2 and our superior hotstart enzyme Cheetah™ Taq. Cheetah™ Taq is a chemically modified hotstart Taq which can be fully activated in 2 minutes, thus permitting fast PCR. This master mix is offered with ROX reference dye and has sufficient reagent for 500 reactions.
Meridian Life Science SensiFAST Probe Lo-ROX Kit, 5000 x 20 μL reactions, TaqMan/Scorpions/molecular beacon compatible, Lo-ROX dye, antibody-mediated hot-start DNA polymerase, 2x mastermix
SensiFAST Probe Lo-ROX Kit, 5000 x 20 μL reactions, TaqMan/Scorpions/molecular beacon compatible, Lo-ROX dye, antibody-mediated hot-start DNA polymerase, 2x mastermix
ABclonal Technology 2X Universal SYBR Green Fast qPCR Mix, 25 mL, SYBR Green I, ABclonal Hot Start Taq DNA Polymerase, Universal ROX Dye
The ABclonal 2X Universal SYBR Green Fast qPCR Mix is a highly efficient solution tailored for sensitive real-time quantitative PCR applications. This ready-to-use mix combines advanced components including a hot start polymerase, optimized buffers, and a proprietary dye, ensuring accurate results across a variety of qPCR instruments. Its formulation minimizes non-specific amplification and enhances specificity across diverse samples.
- 2X concentrated format
- Compatible with all qPCR instruments
- Hot start capability for improved specificity
- Includes SYBR Green I and universal ROX dye
- Demonstrates high reaction efficiency (99%)
- Features excellent freeze-thaw stability
- Recommended for target lengths of 70-200 bp
- Storage temperature of -20℃
- Used for research purposes only
- Protect from light during storage